Creutzfeldt-Jakob disease is a rare, inevitably fatal neurodegenerative condition that causes rapidly progressive dementia. Here we present a case report of a 70-yearold female patient who initially presente with a suspected stroke. Further clinical investigation detected typical features of Creutzfeldt-Jakob disease. The patient’s condition worsened gradually, and she died five months after hospitalization. This case report demonstrates that differential diagnosis of a rapidly progressive dementsia might be rather complex and challenging. Two possible methods of a reliable premortem diagnosis of Creutzfeldt-Jakob disease are also described: real-time quake induced conversion and evaluation of tauprotein’s serum levels.